Ostrich crystallins. Structural characterization of δ-crystallin with enzymic activity
نویسندگان
چکیده
منابع مشابه
Biochemical characterization of crystallins from pigeon lenses: structural and sequence analysis of pigeon delta-crystallin.
Crystallins from pigeon eye lenses were isolated and purified by gel-permeation chromatography and characterized by gel electrophoresis, amino-acid composition and sequence analysis. Alpha- and beta-crystallins could be obtained in relatively pure forms by single-step size-exclusion chromatography whereas an extra step of ion-exchange chromatography was needed for the separation of delta-crysta...
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Interaction among crystallins is required for the maintenance of lens transparency. Deamidation is one of the most common post-translational modifications in crystallins, which results in incorrect interaction and leads to aggregate formation. Various studies have established interaction among the α- and β-crystallins. Here, we investigated the effects of the deamidation of αA- and αB-crystalli...
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L he term "crystallin" applies to the major structural proteins responsible for the refractive properties of the lens." The crystallins generally make up 90% or more of the total protein mass. The crystallins are tightly packed in the lens fiber cells (Fig. 1). In vertebrates, the most common crystallins are the a, /3, and y families. In birds and reptiles, there is another major lens protein, ...
متن کاملAssociation of partially folded lens βB2-crystallins with the α-crystallin molecular chaperone
Age-related cataract is a result of crystallins, the predominant lens proteins, forming light-scattering aggregates. In the low protein turnover environment of the eye lens, the crystallins are susceptible to modifications that can reduce stability, increasing the probability of unfolding and aggregation events occurring. It is hypothesized that the α-crystallin molecular chaperone system recog...
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in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1991
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2730295